Can anyone suggest some keys for identification of marine and mangrove fungi in India?

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Which is the best method for extraction of DNA from Marine Fungi?

I had refered some literature about Fungal DNA extraction. I had seen that CTAB , Phenol chloroform , Kit methods are commonly used ones. So which will best one for extraction of DNA from Marine...

22 October 2015 5,310 2 View

During single spore isolation how many germinating spores are transfered from water agar into PDA?

While transfering germinating spores into pda instead of my desired fungi other fastidious fungi and bacteria is inhibiting the growth of desired fungi. How I can overcome this contamination. How...

18 June 2015 5,897 12 View

How do I overcome contamination during single spore isolation?

Currently I am working on single spore isolation method. some spores are germinating and some are non germinating.When i transfered the germinating spores into PDA they are contaminated with...

17 June 2015 9,170 13 View

Can anyone suggest me a standard protocol for the estimation of a salinity of a sea water?

i have searched protocols for the estimation of a salinity of a sea water.but i need to know the widely used method please refer me good method

25 May 2015 6,788 5 View

Are there any methods to know antimicrobial activity of fungal cultures other than extraction and performing disc diffusion or well diffusion Assays?

It is very difficult to get Fungal Extract from many fungal cultures. I need to know whether any other methods to perform Antimicrobial microbial activity without extracting fungal extract. we can...

02 April 2015 7,559 13 View

Can anyone suggest a good article for Extraction identification and characterization of Secondary metabolites from Fungi?

I studied that ethyl acetate, hexane and methanol are best for extraction process. Are there any methods for identification of compounds apart from GCMS,HPLC,NMR? Can anyone suggest some good...

08 March 2015 5,663 5 View

What are the method used to induce sporulation of Ascomycetes On PDA?

I tried to induce sporulation by using sterile match sticks but thy are not sporulating Why they are Sporulating on Twigs as Host and why they are not Sporulating on PDA invitro

03 March 2015 1,455 5 View

Which solvents can be used for the extraction of hydrophobins and which concentration is require for the extraction?

The literature suggests that we have to add 2% SDS and triflouroacetic acid or formic acid can be used for extraction of hydrophobins. Is there any other solvent which can be used for this...

03 March 2015 8,032 2 View

What are the molecular identification methods used for fungi?

I had studied that internal transcribed spacer (ITS), Isolation of DNA and PCR amplification and DNA sequencing and BLAST and phylogenetic analysis are used. Can anyone suggest articles about...

03 March 2015 5,416 8 View

What are the cheapest and best methods for preservation of ascomycetes for a long time?

I had studied about freeze drying methods, mineral oil, distilled water, sterile soil, Silica gel. Among these methods which will be suitable method for preserving ascomycetes

03 March 2015 8,031 6 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View