I have the whole genomic DNA of 33 leishmania isolates. Those may be of L. donovani or L. tropica. Which region of those DNAa is suitable to amplify for species differentiation? ITS region or some other?
Schönian, G., Nasereddin, A., Dinse, N., Schweynoch, C., Schallig, H. D., Presber, W., & Jaffe, C. L. (2003). PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagnostic microbiology and infectious disease, 47(1), 349-358.
please let me know if this paper is good for you. all the best with your project.