28 Questions 8 Answers 0 Followers
Questions related from Argha Mario Mallick
What should be the final conc of PI used?
12 October 2023 558 2 View
In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?
26 June 2023 4,377 2 View
we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...
14 March 2023 2,485 4 View
What is minimum amount of the 2 proteins we need for getting a correct result ?
21 February 2022 2,724 3 View
There are lots of reviews on combination of drugs for cancer therapy. But nowhere is a good explanation of why these combination of drugs are used. What is the basis of using such combinations?...
15 October 2021 6,322 1 View
Can anyone provide me some reference paper where image stitching is done with a fiduciary marker
11 October 2021 9,800 2 View
I ask for a solution regarding this issue. we did 0.1% gelatin coating. Is it recommended to remove DMSO by centrifuging? Invitrogen technical did not recommend removing the DMSO.Tried culturing...
24 March 2021 7,808 2 View
Injected 10^5 cells subcutaneously in the 4th mammary fat pad. Distinct tumour is not formed rather the whole abdomen portion is getting swelled. What are the things that might have got wrong?...
24 March 2021 1,995 0 View
Can I thaw matrigel by keeping at 4 degree Celsius fridge instead of ice? Will it be detrimental for the matrigel?
05 March 2021 2,628 0 View
The dialysis buffer composition for proteomics analysis of cell lysate to remove Gn-HCl by dialysis
04 March 2021 2,799 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
03 March 2021 4,008 2 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
01 March 2021 546 2 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
01 March 2021 8,018 3 View
Can you please provide me the reference
18 November 2020 6,294 2 View
the cell lines should be economical and authentic. is there any Chinese Japanese Indian or any other company who ships cell lines to India at an economical price? I know about NCCS , anything...
15 July 2020 3,722 0 View
for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell
03 July 2020 10,111 1 View
I do research in Kolkata, India?
07 October 2019 9,558 3 View
The cell lines should be radiation resistant
07 October 2019 7,652 1 View
I want to monitor the tumor size. Does 4t1 needs to be labelled? What are the most common procedures used to monitor tumor size by imaging in a live/anasthetic mice ?
28 March 2019 5,777 1 View
I have seen in some studies that the level of wild type p53 has increased after treatment with DNA damaging agents such as Doxorubicin in MDA-MB-231 . So is there any studies which show the amount...
24 January 2019 3,387 1 View
We are facing a problem in detecting ERK-1 protein expression. We are doing knockdown experiment of ERK-1 protein. We use peptide based vehicle for siRNA delivery and it is FAM labelled which...
12 January 2019 9,955 0 View
Example of time points like 24hrs or 48 hrs or 72 hrs . The drug is Doxorubicin. Cell line used is breast cancer cell line MDA-MB-231
16 October 2018 8,861 3 View
In my lab cells are cultured in DMEM and for certain experiments like to see the intake by cells we use opti-mem media. But my new cell type is being cultured in RPMI-1640 with 1mM sodium pyruvate...
10 May 2018 2,403 3 View
It is well known that P53 causes cell cycle arrest in G1-S and also on G2-M transition. But is there any evidence that P53 is responsible for S-G2 arrest? I have got only 1 paper saying that P53...
06 April 2018 4,970 1 View
What type of problems can I face? For standardizing an experiment is whole antibody preferable over primary antibodies consisting only of kappa light chains? Why such problem arises?
01 February 2018 8,316 1 View
Please refer papers with detailed protocol that can be understood for biology students
08 December 2017 9,904 3 View
Please provide the references. The peptides would be used for therapeutic purposes for humans
21 October 2017 7,029 3 View
mainly for ERK1 silencing
24 August 2017 7,594 2 View
