Can I thaw matrigel by keeping at 4 degree Celsius fridge instead of ice?Will it be detrimental for the matrigel?

05 March 2021 0 3K Report

Can I thaw matrigel by keeping at 4 degree Celsius fridge instead of ice? Will it be detrimental for the matrigel?

Badges
Science topic

More Argha Mario Mallick's questions See All

Why do I experience random death of cells after passaging?

Hello Everyone, I am culturing few different kinds of adherent cells. However, I sometimes experience cells dying in the flask after passaging them in one flask whereas same cells suspension...

07 February 2024 4,354 0 View

How to trypsinize IMR90 cells to get good viability?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

18 January 2024 958 1 View

IMR90 cell viability and immunoflorescence for alpha SMA staining?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

20 December 2023 4,363 0 View

What should be the final conc of PI in apoptosis/necrosis assay with annexin V in flow cytometry?

What should be the final conc of PI used?

11 October 2023 587 2 View

What is the mechanism behind biological nitrogen fixation in green microalgae in axenic medium ? without any bacterial association ?

I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab...

11 August 2023 3,256 2 View

How to pull a small molecule/TM protein from one end of box to another end?

Small molecule is embedded into a membrane. Pulling direction is x-y (plane of the membrane). How to conduct this simulation in AT/CG methods? Wanted to calculate the free energy change during...

25 July 2023 8,684 0 View

Can I culture suspension cell line (Jurkat) in flasks used for adherent cell line?

In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?

25 June 2023 4,412 2 View

Can I calculate optical energy band gap from the absorbance of UV-Vis spectrophotometer or It can only be calculated from the reflectance ?

Can I calculate the optical energy band gap from the absorbance of the UV-Vis spectrophotometer, or It can only be calculated from the reflectance? Thank you

27 March 2023 9,503 1 View

What are few normal cell lines to test toxicity on normal cells of designed anti cancer drug?

we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...

13 March 2023 2,516 4 View

What is derivative of weight loss (%) /temperature. How it can be calculated from the TG-DTA data, and is that possible to get it in negative ?

Hello all the members. I am getting negative values when I have taken the derivative of weight loss(%) w.r.t Temperature, what is this implies ? Thank you

22 February 2023 6,718 0 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Are these iPSC colonies?

I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...

07 July 2024 9,034 2 View

Breaking organoids to single-cell suspension while passaging?

I'm currently working with mouse uterine organoids and I've seen that many authors perform organoid passaging using mechanical or enzymatic methods to dissolve the Matrigel while breaking the...

01 July 2024 381 2 View

What are these clumps in my transfected PBMCs?

Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...

22 June 2024 346 4 View

Is it possible to form a cell monolayer on top of Matrigel within an invasion assay?

I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...

17 June 2024 8,695 0 View

Discuss the potential effects of melting polar ice caps on global sea levels and climate patterns?

Polar ice

12 June 2024 9,997 1 View

Why is iPSC in bad condition?

I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...

12 June 2024 6,087 0 View

What's wrong with my iPSC cell morphology?

This shape gradually spreads. Even if you install new cells again, it will change like this at some point. Has anyone experienced the same thing as me? I tried using Matrigel again by aliquot it....

12 June 2024 7,269 5 View

Our -20 Freezer thawed unexpectedly, can freeze-thaw of K-glu internal solution cause issues obtaining Gigaohm seals?

We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...

06 June 2024 2,234 1 View

Pancreatic cancer organoids not growing well?

Hello everyone, I am currently working on patient derived organoids from pancreatic cancer (PDAC) patients. I noted that my organoids never grow too much. after 1 month in culture they are...

04 June 2024 646 7 View