Can I thaw matrigel by keeping at 4 degree Celsius fridge instead of ice?Will it be detrimental for the matrigel?

05 March 2021 0 3K Report

Can I thaw matrigel by keeping at 4 degree Celsius fridge instead of ice? Will it be detrimental for the matrigel?

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Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Error encountered in executing MCE ?

In Terrset, an error encountered in executing Spatial Decision Modeller (MCE). During execution or run the MCE a massage come on the screen that reflects "Your columns and rows are not same" , I...

07 December 2020 1,542 1 View

I want to synthesize a GUV. Can you tell the lipid composition and ratio that I should use that will mimic the biological cell/endosomal membrane?

Can you please provide me the reference

17 November 2020 6,128 2 View

Can anyone suggest suitable RNA immunoprecipitation (RIP) protocol from cell?

I would like to see RNA-protein interaction following transcription process inside the cell. I am wondering if there is any protocol similar to protein co-immunoprecipitation,which can bind the...

27 October 2020 2,940 3 View

How to do extract Urban Heat Island separately from LANDSAT image for each land classes?

I have already been read many articles and found some methods but those are not cleared to me. Can you please suggest me how to generate different LST map of each land classes using ArcGIS? Or...

25 October 2020 5,978 5 View

Would anyone please suggest any book/guideline on Agent-Based-Models that address human migration dynamics?

I would like to assess how the reasons of migration varies across type of environmental migrants, and therefore, how it could be useful for modelling adaptation strategies for the future migrants.

04 October 2020 5,435 7 View

For how long should a mammalian cell line be kept in -80 degree C after transferring it from liq nitrogen to culture the cell in media?

for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell

02 July 2020 9,912 1 View

Working on cryogenic micromachining and polymer behavior at Tg. Can anyone suggest some book or information source to know deep about this topic ?

1. LATEST RESEARCH TREND ON CRYOGENIC MACHINING 2. POLYMER BEHAVIOR BELOW GLASS TRANSITION TEMPERATURE

23 January 2020 4,482 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

How long can you store Matrigel-coated Transwell inserts?

Hi, everyone! I was planning to do an invasion assay today and thus coated my inserts yesterday, but unfortunately, due to certain circumstances, I cannot proceed. The next time that I can do the...

09 February 2021 8,771 3 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

31 January 2021 2,817 2 View

Should the Ice 1h crystalline structure be lost during equilibration process in LAMMPS?

I have created an Ice 1h structure and am equilibrating it 100K in NVT ensemble in LAMMPS for a timestep of 0.25fs in 3000 steps. The potential used is TIP4P/Ice. Although the simulation runs fine...

26 January 2021 9,603 11 View

How to remove a sapphire disk from LR white resin embedding ?

Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...

13 January 2021 5,223 3 View