Does MDA-MB-231 have some amount of wild p53 along with mutated p53? Does antibody for wild type p53 can distinguise between wild and mutated p53?

24 January 2019 1 3K Report

I have seen in some studies that the level of wild type p53 has increased after treatment with DNA damaging agents such as Doxorubicin in MDA-MB-231 . So is there any studies which show the amount of wild type or atleast confirms the presence of wild type p53 along with mutated p53 in MDA-MB-231?

Kunal Pal

you can go for western blotting to know the status of p53.

Badges
Science topic

More Argha Mario Mallick's questions See All

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Error encountered in executing MCE ?

In Terrset, an error encountered in executing Spatial Decision Modeller (MCE). During execution or run the MCE a massage come on the screen that reflects "Your columns and rows are not same" , I...

07 December 2020 1,542 1 View

I want to synthesize a GUV. Can you tell the lipid composition and ratio that I should use that will mimic the biological cell/endosomal membrane?

Can you please provide me the reference

17 November 2020 6,128 2 View

Can anyone suggest suitable RNA immunoprecipitation (RIP) protocol from cell?

I would like to see RNA-protein interaction following transcription process inside the cell. I am wondering if there is any protocol similar to protein co-immunoprecipitation,which can bind the...

27 October 2020 2,940 3 View

How to do extract Urban Heat Island separately from LANDSAT image for each land classes?

I have already been read many articles and found some methods but those are not cleared to me. Can you please suggest me how to generate different LST map of each land classes using ArcGIS? Or...

25 October 2020 5,978 5 View

Would anyone please suggest any book/guideline on Agent-Based-Models that address human migration dynamics?

I would like to assess how the reasons of migration varies across type of environmental migrants, and therefore, how it could be useful for modelling adaptation strategies for the future migrants.

04 October 2020 5,435 7 View

For how long should a mammalian cell line be kept in -80 degree C after transferring it from liq nitrogen to culture the cell in media?

for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell

02 July 2020 9,912 1 View

Working on cryogenic micromachining and polymer behavior at Tg. Can anyone suggest some book or information source to know deep about this topic ?

1. LATEST RESEARCH TREND ON CRYOGENIC MACHINING 2. POLYMER BEHAVIOR BELOW GLASS TRANSITION TEMPERATURE

23 January 2020 4,482 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View