04 April 2021 0 717 Report

After applying matrigel:pbs 1:1 on cover slip after how much time should we seed the HUVEC on it?

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Why do I experience random death of cells after passaging?

Hello Everyone, I am culturing few different kinds of adherent cells. However, I sometimes experience cells dying in the flask after passaging them in one flask whereas same cells suspension...

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How to trypsinize IMR90 cells to get good viability?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

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IMR90 cell viability and immunoflorescence for alpha SMA staining?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

20 December 2023 4,363 0 View

What should be the final conc of PI in apoptosis/necrosis assay with annexin V in flow cytometry?

What should be the final conc of PI used?

11 October 2023 587 2 View

What is the mechanism behind biological nitrogen fixation in green microalgae in axenic medium ? without any bacterial association ?

I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab...

11 August 2023 3,256 2 View

How to pull a small molecule/TM protein from one end of box to another end?

Small molecule is embedded into a membrane. Pulling direction is x-y (plane of the membrane). How to conduct this simulation in AT/CG methods? Wanted to calculate the free energy change during...

25 July 2023 8,684 0 View

Can I culture suspension cell line (Jurkat) in flasks used for adherent cell line?

In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?

25 June 2023 4,412 2 View

Can I calculate optical energy band gap from the absorbance of UV-Vis spectrophotometer or It can only be calculated from the reflectance ?

Can I calculate the optical energy band gap from the absorbance of the UV-Vis spectrophotometer, or It can only be calculated from the reflectance? Thank you

27 March 2023 9,503 1 View

What are few normal cell lines to test toxicity on normal cells of designed anti cancer drug?

we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...

13 March 2023 2,516 4 View

What is derivative of weight loss (%) /temperature. How it can be calculated from the TG-DTA data, and is that possible to get it in negative ?

Hello all the members. I am getting negative values when I have taken the derivative of weight loss(%) w.r.t Temperature, what is this implies ? Thank you

22 February 2023 6,718 0 View

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