Has anyone tried zymo whole blood RNA extraction kit for mirna. How's the yield and quality of small rna and total RNA
I have used Zymo research kits. All most all are better both in terms of quality and quntity but final decision depends upon end-user requirement.
In a heat exchanger, the cold stream is a corrosive material and it flows through the tube side. The shell side fluid is at a higher pressure than the tube side_ Recently one of the tubes in the...
01 December 2020 2,860 6 View
I am working with raster data for ANN, i have converted rasters to numaric and then test train that numerical data and get very Good accuracy. now i need to convert my train data into raster using...
14 November 2020 9,902 2 View
Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Meteorology Direction related to Hydro Meteorology, remote...
22 June 2020 3,475 16 View
Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Environmenal Research Direction related to remote sensing,...
21 June 2020 1,539 28 View
I made a Lithium sulfur cell, with Sulfur/Carbon cathode and Lithium metal anode using 100 uL of electrolyte (0.6 M LiNO3 and 0.4 M LiTFSI in DME:DOL). I am using a Biologic VSP 300 battery cycler...
27 May 2020 1,232 5 View
Currently, I am using STATA 13 but the graphical output is not as good as presented in papers (meta-analysis) published in some reputable journals.
28 April 2020 7,979 5 View
Any useful software for applying neural networks in power systems? I know MATLAB has a NN toolbox but besides that, is there any other useful software available?
20 April 2020 9,663 16 View
Any suggestions on where to begin learning neural networks for beginners? Some advice on useful resources , books, papers, etc will be appreciated. Thanks
18 April 2020 8,153 13 View
Any suitable index for describing power system frequency stability under a three phase fault?
01 April 2020 9,204 12 View
what is DPL command to find maximum value in a graph drawn in Digsilent Power Factory?
28 March 2020 5,674 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View