Which primer pair is better for amplifying endophytic fungal communities in roots of trees while avoiding host amplification?

Which primer pair is better for amplifying endophytic fungal communities (metabarcoding with Illumina sequencing) in roots of trees while avoiding host amplification? I would greatly appreciate a...

03 September 2020 728 3 View

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Hello, I am looking for the original version of COHIP. To measure quality of life related to oral health, in children.

15 June 2020 5,166 1 View

Whas is the ideal pathway for making an economic assesment for hidrologic ecosystem services?

17 April 2020 5,969 5 View

What is the correct interpretation for this association between changes in two variables?

Hello everybody! We are carrying out a research where we associate baseline to post-intervention changes between motivation and physical activity variables. My question is the following: In an...

24 March 2020 910 3 View

UMBRELLA SAMPLING: How can I calculate dG from a PMF curve in automated fashion?

1. I am processing my umbrella sampling simulations using the Weighted Histogram Analysis Method in Gromacs (gmx wham). However, I would like to report the mean dG and a standard deviation. To do...

15 March 2020 1,455 3 View

How do I calculate free energy of binding for ligand-protein complex using Bennett Acceptance Ratio (BAR)?

Hi, I am familiar with performing MD simulations using Gromacs on protein-ligand complexes. However, BAR is a new technique to me and I need specific clarification of the decoupling sequence...

12 March 2020 3,619 1 View

What are the most obvious difficulties when we expose the scientific findings to the general population?

When we have the opportunity to express our scientific findings in front of our colleagues we have the opportunity to talk with similar people, but; What happens when we have to speak in front of...

13 January 2020 501 4 View

Overlap PCR resulting into smear. Any idea of why?

I want to fuse 2 genes (4kbp each gene). I amplified both of them by PCR adding an overlapping region of 27 bp and gel extracted them. First thing that I don't like is that I can't quantify the...

17 December 2019 6,622 9 View

Where can I get a key to Saturniidae (silkmoths) genera?

I would like to know if there are taxonomic keys, besides the ones in Lemaire's books, for any group within Saturniidae family. UPDATE: Thank you all for your answers, Lemaire's books are indeed...

22 May 2019 2,124 15 View

What are the most important functions to know in SPSS for Psychology?

I am on a short deadline and need to learn how to use spss. The program is very large and I am unsure where I should spend my time the most. Chi-square & T-tests Correlations &...

21 May 2019 9,279 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View