Seeds

The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like... | Review and cite SEEDS protocol, troubleshooting and other methodology information | Contact experts in SEEDS to get answers

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Questions related to Seeds

Need help for this fern?

I need some assistance to ID this Fern I collected in remote Papua New Guinea tropical lowland hill forest at 50-100 m asl. I think it is one of the Dryopteridaceae but I can not put a genus and...

28 June 2024 4,960 2 View

Which other method other than Gas Chromatography can be used in the content determination of Alcohol in Pharmaceutical syrup?

Other than GC any other method might help.

27 June 2024 955 3 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

26 June 2024 9,023 6 View

Root Phenotypes in Arabidopsis Col-0 Under Phosphate Deficiency Conditions not showing up??

Introduction: I am conducting an experiment to investigate the root phenotypes of Arabidopsis Col-0 plants grown under phosphate deficiency conditions. Here, I describe my current methodology and...

20 June 2024 8,634 3 View

Sorghum bicolor accumulates heavy metals?

If Sorghum bicolor is an accumulation of heavy metals, how are they used as fodder and food for animals?

18 June 2024 8,017 1 View

Heterologous gene expression?

Hi, My question will be at a very fundamental level about heterologous gene expression, For any (heterologous) gene we insert between any promoter and the corresponding terminator sequence, is...

15 June 2024 3,928 3 View

How to increase qPCR efficiency to acceptable range?

My aim is to create a standard for qPCR for absolute quantification of DNA extracted from soil. The reaction contains PowerUp SYBR Green Master Mix, BSA, primer pair (A189F-MB661R- 0.67 uM), and...

11 June 2024 6,578 9 View

Temperature Incubation function on the FLUOstar Omega plate reader?

Hi, I've recently started using the FLUOstar Omega plate reader to perform some basic bacterial growth curves. Before putting the plate in I've been trying to use the temperature incubation...

07 June 2024 5,772 0 View

Why am I observing a m/z peak at 372 for the ciprofloxacin hydrochloride hydrate spectra in MS?

The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.

02 June 2024 7,295 0 View

Are there any recent developments in the discovery or engineering of novel biocatalysts for carbon conversion?

Developments in the discovery or engineering of novel biocatalysts for carbon conversion cannot be stopped. we need to know any recent deevelopment in this field.

19 May 2024 5,218 1 View

Which is one way that movement of matter through an ecosystem is different from transfer of energy & energy flows through a food web in an ecosystem?

Which is one way that the movement of matter through an ecosystem is different from the transfer of energy and energy flows through a food web in an ecosystem?

24 April 2024 9,953 0 View

How to grow Chinese camellia from seed?

On February 27, I soaked 2-year-old Camellia sinensis seeds from Krasnodar in a spark biostimulator with water for 12 hours and planted them in soil with an acidity of 4.5-5.0. But none of them...

09 April 2024 6,527 1 View

How to get projections of spin-orbit entangled Jeff = 1/2 and Jeff = 3/2 states of 4d/5d compounds in VASP ?

In VASP orbital projected density of states (DOS) can be plotted without introducing spin orbit coupling (SOC). But how spin orbit entangled Jeff = 1/2 and Jeff =3/2 states can be plotted in DOS...

01 April 2024 5,804 0 View

What is the dosage of application of nanobiofertilizer for soil application and foliar application?

Nanobiofertilizer is reported in some literatures as only samll quantity required compared to traditional ones.

28 February 2024 7,914 0 View

Trapped charge in semiconductor will produce parasitic field. I'd like to know if this parasitic field could do physical damage on crystal lattice ?

Electric field is a presence resulted from coulomb force of the carrier charges. As it does not manifested physically, could electric field do some mechanical damage such as lattice displacement ?

26 February 2024 5,148 2 View

What is the recent developments in microbial fuel cell ?

In microbial fuel cell Which type of electrodes and membranes can be used and what is the material (waste) is taken.

13 February 2024 5,836 1 View

Why ions affect enzyme activity different in differ buffer?

I have tested enzyme activity (b-galactosidase) with cation ions in different buffer and the results differ dramatically. For example, while I used MES buffer calcium acts as an activator but when...

12 February 2024 2,996 2 View

We are searching for an (redox) indicator dye to assess yeast metabolism that works in a pH range from 4 – 7?

We developed a rapid viability test for plant seeds in which yeasts metabolize organic compounds that leach from seeds (dependent on the physiological age of the seed) and thereby reduce our...

02 February 2024 2,764 2 View

Do you think that is intresting to modify a media to better undestand the potential of bacteria to produce organic acids?

I intend to modify a media, to better access de potential of bacteria (Pseudomonas and Bacillus) to produce organic acids in solid medium.

26 January 2024 2,653 1 View

Are TALEs really Xanthomonas & Ralstonia - specific, and are TALEs specific for T3SS only, or they can be translocated by other secretion systems?

Transcription activator-like effectors (TALEs) from the Xanthomonas (and Ralstonia) are proteins with the remarkable ability to directly bind the promoters of genes in the plant host to induce...

24 January 2024 329 0 View

Biochar amount calculation with volume ?

I am incorporating biochar treatment in my experiment. I have container volume of 1.5m*1.5.*1m. The application rate of biochar is 20t/ha (i.e 2kg/m2). I will do a surface treatment for 0-20 cm....

24 January 2024 2,331 4 View

Yuliana Gaona is taking my research ?

Hello Im Yuliana Jiménez Gaona, and I want to shared that the user Yuliana Gaona is taking my research and already associated with her profile ResearchGate...

24 January 2024 1,767 3 View

Someone could help me to solve a difficulty in establish the parameters for Km analysis (Michaelis Menten)?

I'm trying to establish the Km for different substrates for a specific enzyme, however, at every attempt a new doubt arises. This is a new field of knowledge to me, so any suggestion will be...

23 January 2024 8,637 6 View

What is the ideal restriction digestion reaction volume for southern blotting? Can we scale up the volume to 200 ul if gDNA concentration is low?

while working with low concentrated mammalian genomic DNA, can we increase the restriction digestion reaction volume to 200 ul to accommodate the required amount of mammalian genomic DNA for...

23 January 2024 6,824 12 View

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