You can remove the SDS of gel electrophoresis denaturing?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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Why my gel electrophoresis has smearing?

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Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

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Nisha Rajendran

Can you explain in brief ?

Grant Shimamoto

Are you asking if the SDS can be removed from both the gel and the protein while keeping the protein in the gel in addition to returning the protein to its native conformation?

Holjes Salgado‐Lugo

What I mean is: If the compound SDS, Is it possible to remove it from a denaturing polyacrylamide gel?