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I'm currently working in a project in which it would be useful to measure: transmural flux, resistence and potencial difference in mucosa biopsies. I've been reading about this method and as far...

23 September 2020 6,976 1 View

Is there any suitable approach to solve the LCPN method in hierarchical classification?

I have a multi-class problem. I want to solve this problem using the LCPN (Local Classifier per Parent Node) method of heirachical classification. Therefore, I have manually divided the original...

22 February 2020 2,829 3 View

How to implement hierarchical classification using python?

I have a multi-class problem. I want to solve this problem using the LCPN (Local Classifier per Parent Node) method of heirachical classification. Therefore, I have manually divided the original...

18 February 2020 1,147 2 View

How can I define a custom cohesive response to use with contact pairs in ABAQUS?

Hello, I have a problem in which a steel nail is 'embedded' in a wood piece. The head of the nail is in contact with a steel bracket. As I apply cyclic load on the vertical wood 'wall', the...

09 July 2019 8,084 2 View

Is possible to remove a co-author who only participated in the reasoning of a paper but who did not write or analyze anaything?

this is an ethical question. We have a conflict with a co-author who stopped its contributions, he only participated at the beginning of the project. I did all data analysis, article writing and...

04 March 2019 3,207 6 View

Is Newton's third law applicable to biological systems?

I'm writing a review article in which I explain the different mechanisms by which M. tuberculosis reaches a persistent infection by establishing a cross-signal homeostasis with its host, in which...

16 December 2018 8,212 32 View

Are the random primers only for first-strand cDNA synthesis or can you do one PCR too?

I already did my RT, but now I want to do a PCR to replicate all my cDNA, is it possible to do a PCR with the hexamer random Primer as well? I do not find any KIT for PCR that is not with gen...

13 June 2018 6,254 2 View

The clean energies, as the solar energy could be an alternative to the global warming and the contamination caused by the use of petroleum?

In the case of the combustion motor vehicles

08 April 2018 1,829 8 View

International Survey of Clinically Relevant Plant-derived Molecules

Anybody wants to be part of an international survey about plant-derived compounds in clinical trials in different countries? Let me know please.

06 July 2017 399 2 View

Is there a company that sells human renal erythropoietin producing cells?

17 January 2017 1,328 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View