Nisha Rajendran

5 Questions 25 Answers 0 Followers

Questions related from Nisha Rajendran

Problem with Cryosections?

Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...

11 September 2020 4,030 3 View

HnE Protocol for the frozen section of cells encapsulated in hydrogels?

Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...

29 May 2019 1,388 5 View

What is the procedure for performing ELISA on protein pellet extracted from cell lysate?

Hi all, I wanted to perform ELISA (sandwich elisa) on the protein pellet extracted from cell lysate. I would like to know in which buffer, the protein pellet should be resuspended? Thank you Nisha

11 December 2017 9,469 3 View

Which is the best method to sterilize ECM hydrogels?

I have made collagen based hydrogel for regenerative medicine applications. Since the growth factors are incorporated into the hydrogel, sterilization process should not affect the stability and...

04 August 2017 3,483 9 View

Co-culture protocol for SH-SY5Y and U87-MG?

Hi, I am looking for exact protocol for co-culturing both neuroblastoma as well as glial cells. Please help me out Thanks Nisha

19 November 2016 4,969 1 View