Hello all,
I have a few designs that were constructed computationally using Rosetta. Almost all papers using Rosetta design use high-throughout yeast display, encoding the designs in oligo pools, amplifying, and transforming into yeast.
How expensive is the process of getting apparent KDs from this approach to identify if any of the proteins bind to the intended target as a first line screening protocol before employing other experiments like BLI.
It is only a few designs, and I am not testing thousands of designs like many of the Rosetta papers.
I would always be willing to collaborate with experimental groups for authorship.
Hi, I am also working on Rosetta protein design.
BLI itself won't be too expensive, a box of 96 sensors costs $200-500.
But the question is how are you going to express these proteins. If your protein can be expressed with E.coli, It won't cost much. Expressing your protein with the mammalian expression system may cost more.
If you have less 50 designs, directly test binding affinity with BLI may be a good choice.
Hope that helps!
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