Can anyone suggest a reliable protocol for purification of nuclei from yeast cells (Saccharomyces cerevisiae) and preparation of cytosolic fraction for Western-blot analysis? What proteins can be used as references for nuclear and cytoplasmic fractions?
We regularly use the protocol from this paper for preparation of crude yeast nuclei for chromatin analysis:
http://www.pnas.org/content/104/37/14706.full
This publication may be more relevant, as it summarizes various fractionation procedures:
http://onlinelibrary.wiley.com/doi/10.1002/0471143030.cb0307s07/full
We regularly use the protocol from this paper for preparation of crude yeast nuclei for chromatin analysis:
http://www.pnas.org/content/104/37/14706.full
This publication may be more relevant, as it summarizes various fractionation procedures:
http://onlinelibrary.wiley.com/doi/10.1002/0471143030.cb0307s07/full
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