DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

ANSYS APDL RC Beam modelling?

Hello I have modelled RC beam in ANSYS apdl. first create volume, then create keypoints & create a line joining that keypoints to make reinforcement. I am working on thermal analysis, for...

20 January 2021 7,344 2 View

ANSYS APDL tabular entry related?

Hello I want to add thermal convection load on specific area of concrete volume. it asks for film coefficient which is a constant value but the bulk temperature has no. of values that i have as...

18 January 2021 6,627 1 View

What is the best way to maintain constant concentration of a volatile organic liquid in an open experimental set-up?

The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.

27 December 2020 7,325 2 View

How i can input temperature depedent stress strain curve for concrete & steel in ansys?

i am working on thermo-mechanical analysis of RC beam using ANSYS. i want to input temperature dependant stress strain curve in ansys. i tried using elastic > multilinear but a warning attached...

23 December 2020 9,928 2 View

What are some best features that can be added in IoT based pollution monitoring system ?

Actually I am developing IoT based pollution monitoring system and need some ideas which help in developing great product with great features

10 October 2020 5,657 7 View

Can LoRa Technology be used for managing underwater data center ?

As per the latest research, Microsoft finds underwater data centers are reliable, practical and use energy sustainably so how can we enable IoT with LoRa technology in the underwater data center...

05 October 2020 535 4 View

How can I calculate the concentration of nanoparticles being used in UV-visible absorbance scan?

I have taken 1000 PPM of nanoparticles in 1 ml of RO water and pursuing its UV visible spectro absorbance scan. They are showing respective peaks on the baseis of energy transition of electrons...

19 September 2020 6,276 3 View

How can Gold be extracted from mining concentrates?

I work at a precious metal refinery. We extract gold using aqua regia. However, sometimes Gold does not dissolve in AR. Sometimes it precipitates due to cold weather? Is there a way to make sure...

07 August 2020 7,059 3 View

Chemistry behind synthesis of boron nanoparticle?

Hello everyone, I want to know whole chemistry behind synthesis of boron nanoparticle via chemical and biogenic methods. Focussing on why naked boron is not used as I have seen in papers, boron...

05 August 2020 1,195 14 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View