Would 10% FBS cause inhibitory effects on the production of proinflammatory cytokine production in macrophages stimulated with LPS ?

24 May 2022 1 763 Report

I have stimulated thp-1 cells and applied inhibitor but the inhibitor did not work and I am wondering why would this coul happened, could it be because of the FBS ?

Malcolm Nobre

Hello Batoul Shunnar

Yes, probably you may be right. Inhibitory components of FBS could be responsible.10% FBS present in the medium contains enough TGF-β to inhibit macrophage activation. Thus, culturing macrophages in standard FBS-containing medium may be misleading because TGF-β1 inhibits macrophage activation. For more details you may refer to the research article attached below.

https://www.jimmunol.org/content/164/12/6166

Best.

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Science method

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