Batoul Shunnar

3 Questions 1 Answers 0 Followers

Questions related from Batoul Shunnar

Would 10% FBS cause inhibitory effects on the production of proinflammatory cytokine production in macrophages stimulated with LPS ?

I have stimulated thp-1 cells and applied inhibitor but the inhibitor did not work and I am wondering why would this coul happened, could it be because of the FBS ?

24 May 2022 789 1 View

How to calculate the final concentration of DMSO ?

I have an inhibitor which was diluted in 100% DMSO, the stock concentration is 50mg/mL and the stock volume is 200µL. these were diluted in ddH2O to reach 10µM. the question is how to calculate...

30 March 2022 9,615 0 View

What is the best way to deal with the frozen cells before we start extracting?

Can someone help me by solving this issue? I have a cell culture of THP1 cells and I need to extract RNA but the cells are frozen and they have been washed by PBS and 0.1 M BSA is added. The RNA...

11 March 2022 953 2 View