With China's present economic success, will autocratic government again become the accepted model?

More Stanley Wilkin's questions See All

Why do researchers believe data on rodents can be extrapolated onto humans?

This is a constant when proofs are offered for monoamine hypothesis. I read an, on the surface, interesting, technical paper only to discover the data is about mice- although ostensibly the...

11 December 2018 3,848 0 View

Does supply create its own demand?

Ok. An old question put out 200 years ago by Jean-Baptiste Say, and one usually answered with a 'no'. But can you make a reasonable try at arguing 'yes' strongly and convincingly?

07 August 2018 4,387 15 View

The Golden Rule for Teachers?

I came across this on my linked in site. The question really references the Student Centred Approach to teaching. The above concludes that the Golden Rule involves: Putting yourself in your...

07 August 2018 3,717 23 View

To what extent did the USA cause the collapse of the British and French Empires after World War 11?

During the 2nd World War, President Roosevelt's Atlantic Charter had committed the USA to dismantling the British and French empires in Asia, although not to dismantling their own. Roosevelt had...

07 August 2018 7,707 10 View

Do you rely on authority heuristics when considering a subject?

How many believe a judgement or point of view simply because it comes from a professional or from someone endowed with intellectual or political (in its widest sense) authority? If you are told...

06 July 2018 7,230 5 View

Is intelligence overrated?

Although intelligence is purveyed as a requisite quality how does it really help towards success, for example, happiness and quality of life. Is there something to be said for stupidity? Are...

06 July 2018 5,585 90 View

Is academia just a way of presenting knowledge and does it therefore deserve its status?

Academic training is lengthy but often resolves into specific ways to present knowledge and information. This requires ability but does it require great ability. When I did my first degree I...

06 July 2018 8,980 14 View

What is the overall effect of International Students?

Does their presence in host countries, usually in the West, aid the host countries or their own country more? Do millions of students learning and training in other countries aid globalisation? Do...

06 July 2018 3,159 17 View

Have any of your original professors/lecturers done outstanding work in their discipline and are now celebrated?

I wonder how many ever succeeded but remained big fish in a tiny pool, happy to be under half-lit lamps throwing occasional light on them and their work.

06 July 2018 1,480 1 View

Do you believe Foucault was right to hold that madness is merely a construct?

Is madness just an idea developed as a consequence of the constitution of a prevailing notion of rationality, as Foucault examined in Madness and Civilisation. The development of Normalcy, an idea...

06 July 2018 2,262 7 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View