We have run across a tumor cell line with a high-incidence of ulceration formation (leading to early euthanization in tumor-growth experiments). During a conversation with another group the idea of using Matrigel during injections was suggested as a means of preventing this.
Does anyone have any relevant experience with this solution (Subcutaneous injections)?
Alternatively does anyone have suggestions for reducing incidences of tumor ulcerations?
I think it depend more on tumor type. More invasive tumors form ulcers rapidly. Try using less invasive cell lines or use syngeneic mouse models rather than nude mice.
Don't think so - Matrigel is primarily used to avoid dissemination upon injection - I think that the matrix is cleared and replaced by a proper matrix quire rapidly. Ulceration is more tumor-dependent I am afraid. It may be worsenen by the localization as well. Breast cancer (e.g., Mammary fat pad models) tend to ulcerate more frequently/rapidly since the tumor is more likely to be in contact with the ground when the mouse moved and frictions may increase the phenomenon. When s.s.c. grafting on the flank or on the back, the same model (MDA231 for instance) showed fewer ulceration. It may depend on the tumor size as well - normally small tumors are less likely to ulcerate, based upon my own experience. Reducing the number of cells to be injected could be a solution (we gave up the "1 million cells ssc" paradigm in my unit and start with 80-150 000 cells; works fine). I hope this helps?
Best,
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