ABTS stock preparation was prepared by adding ABTS and potassium persulphate.Can we use it immediately?If Cannot why?
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
Hi, I am working on a head FE model and want to try different material types on brain. I read some papers that use Mooney-Rivlin rubber model and wondering if i can add viscosity into it. Is it...
24 July 2024 5,908 0 View
Hi, I run an FE model on LS-DYNA solver, and concerning about the max principal strain contour result in HyperView. When I choose the result type as Strain(t)-Absolute Max Principal the contour...
19 June 2024 7,577 1 View
Hi, I am running a titration of QAC with sodium tetraphylborate using autotitrator from Metrohm. The titrator identifies multiple end point with different ERC value. Would like to know which EP...
17 April 2024 545 1 View
Hi! I am staining iNOS and CD163 in my cells so I am looking for a minus control, expressing neither iNOS nor CD163. But the cell familiar in our lab including panc-1 and 293T seems at least...
04 April 2024 7,855 1 View
I am planning to design a lab practical to extract RNA from different parts of tomato, namely leaf, fruit and root, followed by expression analysis. Can anyone recommend some tissue specific...
01 November 2023 3,523 0 View
According to the book written by Pope, the vorticity equations exhibit material-frame indifference when the flow is two-dimensional and the rotations of the frame are steady. But how can we judge...
15 May 2023 4,791 3 View
Hi! I use UV and photoinitiator to gel my PEG-acrylate hydrogel. But I observe that when the temperature is lower (at night during winter), it's very easy to get pre-gelled before molding, even...
01 March 2023 9,886 1 View
Hello, I am doing whole cell-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process to look for DNA aptamers for the detection of bacteria. I have purchased my DNA...
18 December 2022 2,144 7 View
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
In most colorimetric determinations of methylglyoxal, there is neutralization using saturated potassium carbonate, and perchloric acid is added to the final reaction. I would like to understand...
23 June 2024 2,074 0 View
methylene blue stock solution
19 June 2024 10,030 3 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View
I'm following a paper for their HPLC methodology, but I'm having a hard time understanding how to make mobile phase A. To quote: "Mobile phase A is a mixture containing 0.2% potassium dihydrogen...
13 June 2024 8,321 1 View