Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...

03 February 2021 8,540 2 View

Regarding cement based pore structure. How do you calculate pore volume using electrical conductivity/resistivity measurements?

I am trying to calculate pore size distribution of concrete using electrical resistivity measurements however I am unable to calculate the volume of the pore structure. Can anyone advise on how I...

19 January 2020 8,683 2 View

Does anybody have any resources to understand how polymerization kinetics of random copolymers work? Specifically hydrogels if possible?

I am performing rate of reaction studies on hydrogels with 2 monomers and crosslinker. We can look at absorbance of a single wavelength of light over the course of the reaction through UV-Vis to...

29 October 2019 4,443 2 View

What disease pathologies are you aware of that would benefit from elemental analysis?

Hello, I am currently researching a spectral computed tomography system for medical applications. The system excels in delineating the elemental composition of materials, providing a 3D...

06 March 2019 3,465 3 View

Making antisense probe from topo?

I need to make an antisense probe from a cloned sequence in pcrTOPO4. When I sequenced using the m13R primer, the sequence through a BLAST search come back as Plus/Minus. Just wondering how i...

01 March 2017 3,003 3 View

Lipid extraction for samples stored in ethanol?

Hello, I'm hoping to extract lipids from some shark muscle samples for some fatty acid analyses. All the literature I can find is based on frozen samples, but mine are stored in ethanol - will...

02 March 2016 9,921 4 View

Any recommendations on which macrophage (P388D1 vs RAW 264.7) to use for studying bacterial killing via the oxidative burst?

I have a mutant bacteria that is more tolerant to H2O2 and I would like to test if it is more persistent in the macrophage model? Will either of these cell lines phagocytose and degrade bacteria,...

23 March 2015 9,088 4 View

Can anyone suggest reasons for the appearance of this non-specific band in PCR?

I am working on amplifying the nirK gene from whole community genomic DNA. However I am getting a second band just below my target (500bp) band. The PCR program I am using is below: 94C-5min |...

03 February 2014 7,182 71 View

Great project! How did you go about warming the students up to the rich picturing process, to get them involved?

How did you go about warming the students up to the rich picturing process, to get them involved? I feel that I have never quite cracked the challenge and disbelief from participants in RP...

01 January 1970 1,738 3 View

The use of artificial intelligence in photogrammety for cultural heritage

I'm writing a dissertation on the use of artificial intelligence to enhance the cultural heritage applications of photogrammetry. I was inspired by Yves Ubelman's work in Palmyra (as featured on...

01 January 1970 9,536 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View