Gabriela Kretzschmar

5 Questions 5 Answers 0 Followers

Questions related from Gabriela Kretzschmar

Normalization of data generated with RNA-seq PolyA and Total to use together. What's the best method?

I have RNAseq data made with selection by PolyA and other RNA-seq Total data. I want to join this data to increase the sampling within some subtypes I have few samples. How can I normalize these...

10 April 2023 4,898 2 View

Is it possible to perform RNA extraction with miRVANA kit on blood collected with PAXgene Blood RNA Tube?

Is it possible to perform RNA extraction with miRVANA kit on blood collected with PAXgene Blood RNA Tube, which has an RNA stabilizer? Would there be any additional steps to remove or inhibit the...

27 August 2021 5,459 1 View

How can I add NONCODE as a track in the UCSC genome browser?

Hello. I am performing the analysis of some lncRNAs, and many of them present their structure only in the NONCODE or RNAcentral database. When I try to open the genomic position of these lncRNAs...

18 August 2020 7,593 0 View

Does qPCR with TaqMan custom probes can work with Tm of primer in 50°C and probes 57°C?

I'm developing a MethyLight assay to verify a specific CpG site in a promoter region of my interest gene in rats. Unfortunately, I don't have a lot of choices to design the probe and primers,...

21 June 2019 1,105 3 View

Wich control should I use to normalize in MethyLight assay the methylation percent for rats tissue?

I will perform a MethyLight assay with DNA of brain rats tissue. Wich control should I use to normalize de methylation percent of reaction? We found inventoried controls for humans, but not for rats.

17 May 2019 9,201 5 View