Why we choose RPMI medium for BxPC3 cell culture, and not DMEM medium?

More Fouzia Zayou's questions See All

Should we divide the data among exploratory factor analysis and exploratory factor analysis or is the same data is used for both?

I'm doing a validation study and for construct validity, I'll analyze my data through EFA and CFA. So, the same data should be used in both processes or should we divide the data? And if we divide...

07 June 2023 7,046 5 View

Interested to work on ornamental plants propagation, can anyone guide or want to work in collaboration?

Ornamental plants that enhance not only beauty, clam and peace in surrounding also beneficial for health

02 January 2023 6,181 2 View

What would be considered the minimum length of document (minimum number of words) for training an LDA/SLDA topic model?

I'm having trouble finding references to my question. example : Is a docmuemt of 200 words large enough to perform a topic model analysis with Latent Dirichlet Allocation? Thanks, Fouzia Bourai

08 May 2021 2,927 3 View

I have observed decrease of particle size with annealing in air? I got it repeatedly same. what is the possible explanation for it? References?

For different samples, i got decrease of particle size with annealing. Can anyone explain me about this?

02 May 2021 6,710 16 View

Why precursors and base are heated before mixing in a coprecipitation reaction?

I have gone through the literature survey and found that few people have synthesized mixed ferrites by separately heating the precursors before mixing them. Can anyone tell me why is that? why not...

23 June 2020 1,995 0 View

What do you think about the difference between autism and schizophrenia?

23 December 2018 7,050 18 View

Could cysteine proteases isolated from plants have an anticancer effect ??

Hello colleagues and friends, Proteases are extremely important signalling molecules that are involved in numerous vital processes like apoptosis, cell growth and activation, adhesion,...

10 October 2018 8,753 3 View

Relationship between signaling pathways, sugar and tumor metabolic pathways?

Dear Professor and colleagues: inflammation and cell proliferation induced via activation of nFKappaB leading to an increase in the transcription of pro-inflammatory cytokines (IL-1β, TNF-α,...

26 February 2018 7,213 3 View

Why hypoxic tumors grow up in the presence of oxygen transported by blood vessels (angiogenesis)?

Hello Dear colleges; I) As we know that cancer cells grow up in a hypoxic conditions; "less oxygen, more growth". Morever; Angiogenesis maintain blood flow to the growing tumor tissues,...

16 February 2018 7,768 6 View

What is a simple protocol for the extraction of phenolic compounds from Ficus Carica?

Hello, I am currently working on Ficus Carica plant and I am looking for a simple protocol for Phenolic compounds (phenolic acid) extraction . Could anybody suggest a simple protocol for the...

27 March 2017 5,115 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View