i am not sure what you mean by cell line, is it cancer cells or normal primary cells?
However, here are some general points about using cell lines for biomarker prediction:
- Cell lines are derived from cancer cells or other abnormal cells, so they may not fully reflect normal physiology. Using cancer cell lines to predict biomarkers in normal tissue could be problematic.
- Cell lines that have been passaged extensively in culture tend to accumulate genetic and epigenetic changes over time. This genetic drift means they may not closely resemble the original normal cells they were derived from.
- Immortalized cell lines act differently from normal primary cells, with altered signaling pathways and gene expression. So biomarkers identified in cell lines may not be relevant for normal tissues.
- Cell lines lack the cellular heterogeneity and 3D architecture found in intact normal tissue. This oversimplifies the system and loses potentially important context. On the other hand, some similarities between cell lines and normal tissue remain. Conservative biomarkers found in both could still be predictive.
In normal tissue, a biomarker is a measurable characteristic of a biological system that is indicative of normal function or its response to an external factor like therapeutic intervention.
For instance, in radiotherapy, one could look into functional assays such as DNA double strand break repair, induction of chromosomal aberrations, or radiation- induced apoptosis in exvivo irradiated blood lymphocytes that could be used as predictors of radiosensitivity. Also, a number of IR-induced transcriptional and translational alterations could be studied. The use of cell lines for predicting biomarkers in normal tissue is still a controversy.
You may want to refer to the articles attached below for more information.
https://doi.org/10.3390/jpm11020140
Clin.Oncol. 2013,25, 610-616
Mutation.Res. 2017, 771, 59-84
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