I recently ran a SEC with newly bought AKTA pure 25M. While running the standard maker protein, I noticed the preC pressure and system pressure curves fluctuate: what could be the cause and how to fix it? Thanks in advance!
I have the same problem (fluctuation on a range of ~0.1 MPa with a ~5ml frequency), on two different sec columns (superdex 200 10/300GL or suoerdex 200 increase 10/300GL), with three different running solutions (water, ethanol, phosphate buffer + glycerol)
however, this happens only at low flow rates (0.1-0.15ml/min) but the problem disappears when I run the SEC at 0.5ml/min, whatever the running solution or the column used, with a very stable pressure.
Thus, this looks to me to be linked to the Aktapure 25M than to the column itself.