Sa Chen

3 Questions 8 Answers 0 Followers

Questions related from Sa Chen

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

20 July 2024 2,110 0 View

Why the system pressure showed fluctuating curve in my SEC with AKTA pure 25M?

Hi everyone, I recently ran a SEC with newly bought AKTA pure 25M. While running the standard maker protein, I noticed the preC pressure and system pressure curves fluctuate: what could be the...

22 August 2022 4,269 2 View

How can I purify an antibody from rabbit serum with insoluble antigen?

I am going to do affinity purification of a polyclonal antibody from rabbit, but the antigen (100KD, with GST tag) is insoluble. Does anyone has experience in purify the antibody with insoluble...

29 September 2015 1,679 6 View