I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted cells, I am attaching pic.
Dear Evangelia Flampouri thanks for reply, Yes , this is mother flask, after trypsinization i split cells half in mother flssk and half in new flask. These might be cells which doesnot deattched while trypsinization ? This can effect morphology of cells?