Kavita Rana

9 Questions 20 Answers 0 Followers

Questions related from Kavita Rana

Problem in growing aspergillus oryzae mtcc-3107?

I am reviving aspergillus oryzae from lyophilized stock (first time) on czapek yeast agar at 26 degree. Its growing as light brown smooth and shiny rather than powdery or fuzzy appearence even...

17 November 2021 4,118 2 View

I am new to western blot, i want to check nfkappa-b p65 protein in cell, i ran western blot, i have some doubts, regarding my results?

I am checking effect of my compound on breast cancer cells, i ran western blot, i am attaching photo, and i have following questions- 1- i have not used phosphatase inhibitor in sample preparation...

14 February 2020 2,848 3 View

I got diffused bands in western blott? What could be the reason?

I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-

05 February 2020 4,657 3 View

Why sometimes mdamb231 cells grows in patterns like rows in-between in few places in flask??

I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...

14 October 2019 2,696 4 View

i want to isolate proteins( related to apoptosis) from breast cancer cells for western blot, how to make cell lysate for maximum protein yield???

I am new to western bloting, i need to isolate protein ( bcl2/ bax/ nf kappa b/ caspase) by western blotting from breast cancer cells, i will use ripa buffer with protease inhibitor cocktail , do...

10 September 2019 2,701 2 View

Can we investigate Ic50 of drug without knowing its drug loading capacity in case of drug prepared with polymer?

I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...

19 December 2018 1,169 4 View

If i have protocols for tissue samples (kidney/liver) viz gsh,SOD,protein can i use them for cell culture(breast cancer cells)???

I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?

01 June 2018 5,723 4 View

If i have protocols for tissue samples (kidney/liver), can i apply those protocols for cells (mammalian cancer cells)???

I have worked on invivo toxicity of liver n kidney, now i m shifting towards invitro toxicity of mamallian cancer cell(breast cancer). I just wanted to know how to standralize the protocols i...

01 June 2018 1,131 3 View

Regarding quantitative (turbidimetry) assay for CRP.

I AM OPTIMISING TURBIDIMETERY ASSAY FOR THE DETERMINATION OF CRP, I USED VARIOUS PROTOCOLS, WITH 200NM CARBOXYLATED LATEX PARTICLES AND POLYCLONAL ANTI-CRP ANTIBODIES, WITH DIFFERENT BUFFERS, BUT...

01 January 1970 4,244 0 View