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Questions related from Kavita Rana
I am reviving aspergillus oryzae from lyophilized stock (first time) on czapek yeast agar at 26 degree. Its growing as light brown smooth and shiny rather than powdery or fuzzy appearence even...
17 November 2021 4,061 2 View
I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-
05 February 2020 4,599 3 View
I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...
14 October 2019 2,622 4 View
I am new to western bloting, i need to isolate protein ( bcl2/ bax/ nf kappa b/ caspase) by western blotting from breast cancer cells, i will use ripa buffer with protease inhibitor cocktail , do...
10 September 2019 2,649 2 View
I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...
19 December 2018 1,119 4 View
I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?
01 June 2018 5,678 4 View
I have worked on invivo toxicity of liver n kidney, now i m shifting towards invitro toxicity of mamallian cancer cell(breast cancer). I just wanted to know how to standralize the protocols i...
01 June 2018 1,059 3 View
I AM OPTIMISING TURBIDIMETERY ASSAY FOR THE DETERMINATION OF CRP, I USED VARIOUS PROTOCOLS, WITH 200NM CARBOXYLATED LATEX PARTICLES AND POLYCLONAL ANTI-CRP ANTIBODIES, WITH DIFFERENT BUFFERS, BUT...
01 January 1970 4,207 0 View