Kavita Rana

6 Questions 15 Answers 0 Followers

Questions related from Kavita Rana

I got diffused bands in western blott? What could be the reason?

I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-

05 February 2020 4,581 3 View

Why sometimes mdamb231 cells grows in patterns like rows in-between in few places in flask??

I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...

14 October 2019 2,602 4 View

i want to isolate proteins( related to apoptosis) from breast cancer cells for western blot, how to make cell lysate for maximum protein yield???

I am new to western bloting, i need to isolate protein ( bcl2/ bax/ nf kappa b/ caspase) by western blotting from breast cancer cells, i will use ripa buffer with protease inhibitor cocktail , do...

10 September 2019 2,633 2 View

Can we investigate Ic50 of drug without knowing its drug loading capacity in case of drug prepared with polymer?

I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...

19 December 2018 1,103 4 View

If i have protocols for tissue samples (kidney/liver) viz gsh,SOD,protein can i use them for cell culture(breast cancer cells)???

I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?

01 June 2018 5,662 4 View

Regarding quantitative (turbidimetry) assay for CRP.

I AM OPTIMISING TURBIDIMETERY ASSAY FOR THE DETERMINATION OF CRP, I USED VARIOUS PROTOCOLS, WITH 200NM CARBOXYLATED LATEX PARTICLES AND POLYCLONAL ANTI-CRP ANTIBODIES, WITH DIFFERENT BUFFERS, BUT...

01 January 1970 4,197 0 View