Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes?
and is there any method to return them back to life ? specially if the culture produce anamorphic stage ?
and what is the best preservation methods, also some died when stored at -80C in 10% glycerol after few months!!
Thanks in advance.
Sunil Kumar Deshmukh
You can use 50% glycerol in medium and cool gradually maybe 1 degree per minute this may help in long-term preservation or else you can transfer the growth in sterile water and store at 15-20 degree centigrade it will survive for more than a year.
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