Hi Alessandra Façanha Bezerra

I usually coat the glass coverslips with an adhesion factor - The protocol I follow for the suspension cell lines I work on is:

This protocol gives me ~80% attachment of cells - If you use small coverslips as I do, you can optimise your experiments in a 24-well plate and save resources too!

Good luck

Hi,

You can also try to coat your glass coverslips with poly-L-Lysine or with gelatin (0.2%). Both may work and are quite cheap.

Good luck

Coating with poly-lysine is common also. I used 8-well glass slides from a company, they worked very well for MSCs characterization. Please see the reprint. You can put different antibodies on one slide in different wells