Detection of spores in Non-sterile products?

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Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

What is Random Audit?

HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...

31 July 2024 5,309 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How we can use lattice-based cryptography for construction of S boxes?

Please suggest basic literature on lattice-based cryptography. A kind response from the cryptographic community will be highly appreciated.

24 July 2024 6,291 4 View

I am working on III-V based tandem solar cells.Can anyone explain that solar cells work under forward or reverse biased conditions?

I want to know that n-doped side of solar cell is connected with positive or negative electrode.

20 July 2024 1,348 2 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

Phosphorylation sites prediction?

Hello, Does anybody have idea about any software or database that can give information about the possible phospho sites located in a protein? I am trying PhosIDP but its not working or loading...

15 July 2024 1,940 3 View

Hello, does anyone know how I can get coordinates of two specific points on a moving body in Abaqus when applying a vdload subroutine?

Hello I am trying to simulate repulsive force of two permanent magnets in Abaqus. I have implemented Force equations into a vdload subroutine. sensitive parameters are the center to center...

15 July 2024 3,491 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does autoclaving reduce the concentration of heavy metals in sewage wastewater?

I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...

10 June 2024 2,579 4 View

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method?

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method other than cryopreservation of spore for a short term...

10 June 2024 4,992 1 View

What is the correct way to measure the lengths of curved spores?

Measuring the lengths of curved spores sometimes makes confusions. Here in the attached image I have marked two paths (i, ii) the length can be measured. Hope an expert can suggest the correct way.

08 June 2024 1,109 5 View

Dear， How to define sexual spore?

I found it very difficult to define it precisely. Microspore and megaspore of ferns or seed plants are sexual spore? Because the fertilization happened for a long time before the formation of...

27 April 2024 3,077 0 View

Is it necessary to sterilize soil samples before preparing contaminated soil for experiments on degradation of organic pollutants in soil by PMS?

Is it necessary to sterilize soil samples before preparing contaminated soil for experiments on degradation of organic pollutants in soil by PMS? A review of the large amount of papers shows that...

24 April 2024 1,417 2 View

How to aspirate using a microinjector?

I only have a microinjector available to me Femtojet eppendorf ( I am new this and have never used the instrument). I would like to create a negative pressure and aspirate spores. The idea is not...

01 April 2024 5,883 1 View

How do you sterilize olive oil to gavage to germ free mice?

Hello, I am looking for a protocol to sterilize olive oil that will then be suitable to gavage to germ free mice. Importantly, if it were to be dry-heated, could it pose a fire risk?

28 March 2024 3,760 1 View

Dear RG community, can you help with the identification of this NPP fungal spore ?

It was found in marine sediments in the Alboran Sea during the Pleistocene. Septate, brown, around 32x15 micrometers, with perforations scattered across the surface, and two apical pori.

26 March 2024 9,603 1 View

Is this FBS debris in my cell media?

Dear all, I prepared my cell media (EMEM+10%FBS) and another cell media (EMEM+2%FBS+1% Pen/Strep). Incubate the aliquot of the media in 37°C for a few days and saw these. They are not moving and...

24 March 2024 7,496 3 View