Why should we do sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1?

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Bruce Pratt

Depends on what information you want.

If you only want to know the amount of active TGF beta, then don't acid activate before the ELISA.

If you want to know the total amount of TGF b (latent + active) then activate before ELISA

Khushali Upadhyay

What is the difference between Latent and Active TGF beta ?

See http://www.ncbi.nlm.nih.gov/pubmed/18818372 and http://en.wikipedia.org/wiki/TGF_beta_Activation , and references there-in

Thank u so much.. Pratt.