Which method is good for extract preparation?

I am working on one plant for that I require to prepare methanolic and aqueous extract of leaf. Which method I have to use for that? I have to do cell line testing as well as the characterization...

31 December 2019 6,142 3 View

What is the Short term effect of Antiestrogenic Molecular treatment on TGF beta level in case of MCF-7 Cells ?

If we treat MCF--7 cells with Tamoxifen and 4-OH Tamoxifen drug for 5days. and then we are going to check a TGF-Beta level in this cells and compare with non treated cells. Dose it make any...

03 April 2015 4,839 1 View

On the MCF-7 cell growth, what is the effect of complete growth medium (especially EMEM) with phenol red and with out phenol red ?

We have got less cell confluency (60%) in case of With out phenol red EMEM . WE can not use with phenol red EMEM media as we are performing the effect of Tamoxifen drug on Estrogenicity of cell...

02 March 2015 7,639 6 View

Why should we do sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1?

Why we should do Sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1 ??

01 February 2015 2,855 4 View

Are there any problems if we use media EMEM without Phenol red for the MCF-7 cell line and In Vitro Cell culture?

We are going to perform growth curve , cytotoxicity assay,protein estimation,ELISA, Anti cancer drug (Tamoxifen) effect on cell population and Soft agar colony Assay. We are using without...

01 February 2015 5,740 12 View

How can I compare ELISA Techniques for growth factor TGF beta 1 estimation?

We will going to perform Estimation of Elevated level of cellular TGF beta 1 in MCF-7 cell line after giving the treatment of Tamoxifen (10^-6M). For that we are choosing ELISA technique.(Indirect...

01 February 2015 218 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View