Dear Khushali, you can see the results in a qualitative approach for TFG-Beta1 estimation e,g Western Blot.  But I suggest you quantitative measurement of the cytokine using quantitative ELISA. Of course for better methodological parameters (better sensitivity, better work of range, better specificity,...), I suggest you sandwich type ELISA for TGF-beta1.

Many companies produce high quality sandwich type direct ELISA kit for TGF-beta1:

For example below companies can offer you good quality Direct Sandwich type ELISA kit for TGF-beta 1:

Diaclone

R&D system,

Cusabio

Abcam

Biolegend

Ray biotech

.....

regards

mehdi

Thank u Sir.

I agree with Mehdi Hedayati. Moreover, we have used the ELISA kit from R&D Systems with excelent results.

Good luck

I agree with Miguel Pericacho, R&D system ELISA kits are very reliable and using them it is possible to estimate total and active form of TGFbeta.

Hello

i agreed with the tower above.

See the attachments 

http://www.rndsystems.com/product_detail_objectname_elisa_assay_product.aspx