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Questions related from Khushali Upadhyay
I am working on one plant for that I require to prepare methanolic and aqueous extract of leaf. Which method I have to use for that? I have to do cell line testing as well as the characterization...
01 January 2020 6,237 3 View
If we treat MCF--7 cells with Tamoxifen and 4-OH Tamoxifen drug for 5days. and then we are going to check a TGF-Beta level in this cells and compare with non treated cells. Dose it make any...
04 April 2015 4,895 1 View
We have got less cell confluency (60%) in case of With out phenol red EMEM . WE can not use with phenol red EMEM media as we are performing the effect of Tamoxifen drug on Estrogenicity of cell...
03 March 2015 7,698 6 View
Why we should do Sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1 ??
02 February 2015 2,911 4 View
we have performed MTT assay for Dosage confirmation of Tamoxifen and 4-OH Tamoxifen in MCF-7 Cell line. But we cant get better results in 5 times performance.So please help me. Give me suggestion.
02 February 2015 8,641 1 View
We will going to perform Estimation of Elevated level of cellular TGF beta 1 in MCF-7 cell line after giving the treatment of Tamoxifen (10^-6M). For that we are choosing ELISA technique.(Indirect...
02 February 2015 284 5 View
We are going to perform growth curve , cytotoxicity assay,protein estimation,ELISA, Anti cancer drug (Tamoxifen) effect on cell population and Soft agar colony Assay. We are using without...
02 February 2015 5,803 12 View
