Hi all,
in several studies focusing on Epilepsy they pretreat mice/rats 30min with scopolamine methyl bromide (1mg/kg) before trating the animal with Pilocarpine.
Most of them used intraperitoneal (IP) injections.
My question is:
why do they wait such a long time?
Normally drugs injected IP are adsorbed pretty fast, like 5min.
Is it just to be on the safe side before the application of the high dosage of Pilocarpine (to induce the seizures)?
Even if this is the case, wouldn't 10-15min be already more than enough?
So far I was not able to find an answer.
Alberto Arboit although the absorption half-time of substances delivered IP in rats is usually on the order of minutes, maximal plasma values can be significantly longer. This depends on the biokinetics of the substance itself, as well as the solvent used for injection. For example, the maximum plasma concentration of IP sulphafurazole in the rat occurs after 60 min! Ref: https://www.sciencedirect.com/science/article/pii/B9780444818713500102?via%3Dihub
So, for scopolamine a time of 20-30 minutes is recommended likely for its metabolic profile in the rat.
Hope this helped
Read this paper, you may get some insights;
Article Differences in Evolution of Epileptic Seizures and Topograph...