We are planning to collect saliva from rats for protein analysis. The issue is that the amount secreted is very low, which makes it difficult to analyze. To increase salivation, we purchased Pilocarpine hydrochloride (Sigma P6503).

Please advise how to dissolve pilocarpine in water or saline (concentration) and the dose to inject IP in each rat. Then, what is the best method to collect the secreted saliva for protein analysis?

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