What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

For a PCR the volume of each component is standard ?

for example the total volume is 50microliters how can we know that what will be the amount of template , buffer, enzyme, primers (forward and reverse), dNTPs, MgCl2 and water?

02 January 2021 3,352 6 View

How can we know about the final concentration of each component to be used in the master mix for the PCR ?

Final concentration for each component in master mix depends on what or how it will be known that in this master mix the concentration required is __ microliters ?

02 January 2021 5,764 2 View

How can I separate features and the background to measure pixel intensity?

For image processing, I am using ImageJ. I need to separate the features from the background and calculate pixel intensity of features and background. Can anybody help me in this? Thanks

09 December 2020 7,472 9 View

Which are the appropriate metrics can be used to calculate overall Precision, Recall and F-measure in different data samples? and when can use F1?

If we have three different domain of data (e.g. security, AI and sport) and we did 3 different case study or experiments (1 for each domain) and we estimate the Precision, Recall and F-measure for...

07 December 2020 9,833 3 View

How can stem cells repair damaged cells?

How can stem cells repair damaged cells? Could anyone provide papers show the therapeutic potential of stem cells in vitro (on the disease model)?

29 November 2020 9,534 6 View

How can to calculate the Computational complexity/cost (espatially, number of operations and space/memory cost) for an algorithm?

As we know, computational complexity of an algorithm is the amount of resources (time and memory) required to run it. If I have algorithm that represents mathematical equations , how can estimate...

25 November 2020 6,950 7 View

Which is the best scale available to measure conflict management styles?

I have come across multiple instruments for conflict. Now I'm confused as to which one to use. Expert opinions are welcome.

22 November 2020 3,651 5 View

Chi square test for the over time tested variable ?

16 November 2020 8,291 4 View

Help me find the transfer function of the following non linear coupled ODE system ?

I need to find the transfer function for the system in MATLAB. I do know the ss2tf command but its not that simple. Please guide.

08 November 2020 4,414 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View

How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

Can someone help me identify the bands?

The samples are replicates (4) of total RNA from HEK-293 T cells. The ladder is a 1kBp ladder on a 1% native agarose gel buffered with 1x TAE. From my searches, there should be two sharp bands at...

15 January 2021 4,712 4 View