Fatima Javeria

5 Questions 8 Answers 0 Followers

Questions related from Fatima Javeria

For a PCR the volume of each component is standard ?

for example the total volume is 50microliters how can we know that what will be the amount of template , buffer, enzyme, primers (forward and reverse), dNTPs, MgCl2 and water?

03 January 2021 3,523 6 View

How can we know about the final concentration of each component to be used in the master mix for the PCR ?

Final concentration for each component in master mix depends on what or how it will be known that in this master mix the concentration required is __ microliters ?

03 January 2021 5,916 2 View

What is the intensity of light, required for plant tissue culture?

Intensity of light required for plant tissue culture?

20 October 2020 6,320 6 View

Why replication occurs from 5' to 3'?

Why replication process do not proceed from 3' to 5'?

19 October 2020 6,345 3 View

Why we use 70% ethanol in plant tissue culture for sterilization/washing?

70% ethanol is used for sterilization and washing of sample in plant tissue culture, why not more or less than 70%?

15 October 2020 9,626 5 View