I am using Polyst. Flat bot. high Bind. MP blank (Costar) micro-plate for making ELISA (HIV ), when i am using this blank plate for ELISA assay , it is found reactive with positive control while all positive samples are non reactive. Why?
It's not really enough information. Why do you assume your "positive samples" are really positive? If the positive control worked, maybe the samples are negative.
Dear Adam
Hello, and thanks
Actually I am in quality department of an IVD company. And I am conforming that the blank micro-plate (as row material) is non-reactive with the positive serum sample or positive controls or negative serum samples, before it is being used by production department for further processing to manufacture the specifically coated ELISA plate. So, I am performing particular ELISA assay with uncounted or blank micro-plate. and if it is found non reactive or negative during the assay than it is consider as "ok" by quality check.
Please tell me if you require any more information.
So the problem you are having is that you are getting a signal with the positive control, when you expect no signal?
Is this supposed to be a sandwich ELISA, but you are leaving out the immobilized antibody? If so, do you block the plate before adding the positive control? If not, the antigen will stick to the plate and it will be detected by the second antigen-specific antibody.
I am agree, it is possible because my positive control control contains virus antigen.
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