What will be size of my expressed protein If my construct contain Mut. Htt96Q linked with BiRA* ?

08 August 2018 1 4K Report

My construct contains Htt 96Q followed by BirA* with a 6 amino acid linker between them. Than what will be size of my expressed protein if molecular mass of BirA* is 35.2 KDa? Based on amino acid calculation it should be 56 KD . But western-blot analysis says approx 72-73KD protein. Is it due to presence of poly Q in protein?

Neeraj Sharma

Thanks Goran, Yes I didn't find any band in negative control during western blot analysis.

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