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Questions related from Neeraj Sharma
My cloned gene contain a signal sequence at N terminal . How can I check the nucleotide sequence is a correct signal sequence. The following uppercase letter are signal sequence cloned in...
02 February 2019 7,651 2 View
I want to add AviTag with my protein at N and C terminal and to express it in mammalian cell line.
10 October 2018 6,727 0 View
I am planing to get two gene together for cloning purpose. My plan is first amplify the gene "x" using a set of primer which will amplify gene x without stop codon. I will amplify another gene "y"...
09 September 2018 6,716 1 View
Can I preserve my Transfected HEK-293 Cells IN -80 degree for few days, Later these will be being used for Microsome Isolation?
09 September 2018 8,576 1 View
My construct contains Htt 96Q followed by BirA* with a 6 amino acid linker between them. Than what will be size of my expressed protein if molecular mass of BirA* is 35.2 KDa? Based on amino...
08 August 2018 3,703 1 View
I am using knockout HEK 293 cell.
07 July 2018 4,496 2 View
Why I found colony for negative control on selection plate (contain only double digested vector : one blunt (by EcoRV) and one sticky (by SalI) end with ligation mixture, no insert, after...
07 July 2018 340 1 View
I want to ligate my 1kb insert (PCR product) with 6 kb vector, both are digested by ECORV (creating blunt end) and Sal1 (creating sticky end). How? Is blunt end may create problem in cloning?
06 June 2018 6,808 3 View
I am using Polyst. Flat bot. high Bind. MP blank (Costar) micro-plate for making ELISA (HIV ), when i am using this blank plate for ELISA assay , it is found reactive with positive control while...
01 January 2017 5,780 6 View