Neeraj Sharma

9 Questions 7 Answers 0 Followers

Questions related from Neeraj Sharma

Is my signal sequence is correct or functional?

My cloned gene contain a signal sequence at N terminal . How can I check the nucleotide sequence is a correct signal sequence. The following uppercase letter are signal sequence cloned in...

02 February 2019 7,652 2 View

Could any one please provide me a mammalian expression vector with AviTag to add at C and N terminal of protein ?

I want to add AviTag with my protein at N and C terminal and to express it in mammalian cell line.

10 October 2018 6,729 0 View

Can I get single fragment using two PCR product in single reaction ?

I am planing to get two gene together for cloning purpose. My plan is first amplify the gene "x" using a set of primer which will amplify gene x without stop codon. I will amplify another gene "y"...

09 September 2018 6,717 1 View

Can I preserve my Transfected HEK-293 Cells IN -80 degree for few days, Later these will be being used for Microsome Isolation?

09 September 2018 8,577 1 View

What will be size of my expressed protein If my construct contain Mut. Htt96Q linked with BiRA* ?

My construct contains Htt 96Q followed by BirA* with a 6 amino acid linker between them. Than what will be size of my expressed protein if molecular mass of BirA* is 35.2 KDa? Based on amino...

08 August 2018 3,704 1 View

How long do I need to treat transfected HEK 293 cells with Brefeldin A and the concentration need to see endo H resistance in glycosylated protein ?

I am using knockout HEK 293 cell.

07 July 2018 4,497 2 View

Why I found colony for negative control (contain only double digested vector : one blunt and one sticky end)after transformation in DH5-alfa cells ?

Why I found colony for negative control on selection plate (contain only double digested vector : one blunt (by EcoRV) and one sticky (by SalI) end with ligation mixture, no insert, after...

07 July 2018 342 1 View

I want to ligate my 1kb insert (PCR product) with 6 kb vector, both are digested by ECORV (creating blunt end) and Sal1 (creating sticky end). How?

I want to ligate my 1kb insert (PCR product) with 6 kb vector, both are digested by ECORV (creating blunt end) and Sal1 (creating sticky end). How? Is blunt end may create problem in cloning?

06 June 2018 6,809 3 View

Why Polyst. Flat bot. high Bind. MP (Costar) blank micro-plate is reactive with positive control?

I am using Polyst. Flat bot. high Bind. MP blank (Costar) micro-plate for making ELISA (HIV ), when i am using this blank plate for ELISA assay , it is found reactive with positive control while...

01 January 2017 5,781 6 View