Hi,

I am working on a zebrafish model and trying to induced stress using copper sulfate. Although there is a very limited number of publications, I have found that experiments were performed at a 10uM-200uM concentration to induce stress/inflammation in larvae. However, I found that even at the lowest concentration (10um) the larvae survived only for 1 hour.

Also, the solution became turbid (Bluish).

I want to know why the solution is getting bluish turbidity? Is the concentration (10uM) is high for copper sulfate? Where I am getting wrong?

Kindly provide your suggestions or related research articles.

Thank you