I was running sds page 8% gel and it started running tilted(see the picture attached). Can anyone tell what can be the reason behind gel running like this?
The fungus i am using is Mucor hiemalis and i am doing layer by layer coating using chitosan and pectin on LDPE film. I want to see antifungal activity (inhibition zone) of the coated film. The...
03 December 2019 1,825 1 View
I am working on marker assisted backcross breeding in rice and during the foreground selection I can see the band in donor as well as recipient parent although it is of different band size. The...
24 April 2019 6,946 3 View
Formally, a computer program should be able to scan such an image, perform image processing or any specific treatment on it and produce the followings. 1) The info about multiple geometrical...
09 May 2018 2,390 4 View
Over past few days, while teaching my students, I realized the paradox (individuals paradox, my name for it) in medical teaching, training and management. While our entire research and training is...
05 April 2018 8,209 3 View
I performed a genomic DNA prep today and my 260/280 ratio is 2.10. Is there a way to clean this up such that I don't have to redo the entire protocol (I don't have enough samples at the moment)?...
07 June 2017 1,088 2 View
I tried many, but pigments are not extracted
17 February 2016 4,742 4 View
This is related to corrugated sheeting industry
12 September 2015 9,740 3 View
I have been trying to transfect siRNA in SHSY5Y cells using lipofectamine 2000 but could not find knockdown of my protein of interest.
12 February 2013 3,451 1 View
I intend to study a protein (X) in context to its role during T Cell activation. It doesn't matter whether it is Th1 or Th2. I need some suggestions like the kind of mitogen, chemically induced etc.
05 November 2012 710 6 View
Tribal populations at high altitude provide an interesting epidemiological window to study human evolution and adaptation. Is research in these un-reached areas still less explored thereby...
01 January 1970 7,920 3 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...
03 March 2021 8,237 3 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View