Is theoretical framework applicable in all research?

More Manita Kaur Virdi's questions See All

How to avoid errors while doing qpcr?

I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...

07 June 2024 7,292 1 View

Is this mycoplasma contamination?

I had mammalian cancer cell line cultured and fixed for confocal imaging. While observing I found these particles around cell nuclei stained with dapi. I checked the main culture for bacteria but...

15 May 2024 9,102 2 View

Is this mycoplasma contamination?

I had mammalian cancer cells cultuted and fixed on confocal slides. I could see these small small bodies stained with dapi. The main culture flask doesnt show any bacterial growth. Is this...

15 May 2024 6,040 3 View

Why my sds gel is running tilted?

I was running sds page 8% gel and it started running tilted(see the picture attached). Can anyone tell what can be the reason behind gel running like this?

17 January 2024 7,779 0 View

Are these skov 3 cells healthy?

I revived 2 skov3 cell lines together in a t-25 flask. These were stored in -80 for more than 6 months. Their morphology is changed. They look stressed. But can anyone what can be the cause of stress?

22 December 2023 6,799 0 View

A theory help you predict and examine but which factors affect your outcome?

theoretical framework and conceptual framework?

28 November 2023 9,306 0 View

How long can we keep pcr in storage before using it for dpnI digestion?

I am trying create a mutant plasmid using DpnI digestion. I had put pcr using the mutant primers. But then as I could not do immediate transformation, I kept it to store in -20 degree. Now if I...

08 September 2023 3,621 1 View

My antibody is not working. What should i do?

I am trying to develop my western blot using an antibody targeting a 17kda protein. It is binding non-specifically and not giving any band(like in the photo). What does it mean is happening? How...

22 August 2023 3,472 4 View

My cryopreserved cells are not reviving. what should i do?

I tried to revive a2780 cells cryopreserved in -80 degrees. Its been more than 12 hours and its still not attaching. What could possibly be happening? What should I do?

21 August 2023 848 4 View

Gc content of my primers is 41%, will they work? if not then can I use gc enhancer?

I designed primers which have the tm 69 is and the gc content is 41%. I tried increasing gc content upto 50% but then its increasing primer tm to 72. If I keep gc content 41%, will my primers...

29 June 2023 7,210 2 View

Is expert panel indispensable for pedagogical framework & scale development?

Hello everyone, Thank you for your time and patience in advance! I attempt to develop a pedagogical framework and a competence scale for my PhD project. I have developed the drafts of both. I...

06 May 2024 2,239 3 View

Compare the post modem perspective by Savikas career construction theory and the African perspective systems theory as framework for career choice?

compare the post modem perspective by Savikas career construction theory and the African perspective systems theory as framework for career choice.

10 April 2024 2,407 0 View

Where does conceptual framework/theoretical framework fall in a grounded theory (Strauss and Corbin)?

I am writing my research using the GT of Strauss and Corbin. However, typical research templates always have this Conceptual or Theoretical Frameworks.

09 April 2024 3,076 1 View

How do I revise the title of an article I on Reserach Gate?

Hi, My recent publication "Exploring cross-boundary collaboration for youth mental health in Sweden – a qualitative study using the integrative framework for collaborative governance". Is wrongly...

27 March 2024 3,153 2 View

Does anyone have access to literature supporting the idea that there are differences in energy demand across different levels of a building?

I'm working on the theoretical framework of my thesis and I can't find literature to support the idea that at different floor heights, there may be differences in energy demand. If someone can...

25 March 2024 4,998 1 View

Ecosystem policy roadmap how to creating the process?

Topic research : Ecosystem policy roadmapping Author : Leonardo Augusto de Vasconcelos Gomes About in my course subject innovation and technology management, My professor assign this research, I...

23 March 2024 8,707 1 View

What theoretical framework is appropriate for ensuring substantive representation of women parliamentarians?

I aim to explore the level of substantive representation among women parliamentarians, acknowledging the diversity within the female demographic and the prevalent male dominance within political...

22 March 2024 8,323 0 View

If the correlation is very less or negative in research framework in SPSS tool then what it indicates?

is there any mistake in the data or there is a probability of no connection between the variables? Kindly help me with the interpretation. Also, explain me the R square as well. I have attached...

18 March 2024 8,311 3 View

Is there a theory about the study of AI?

theoretical framework

10 March 2024 2,091 3 View

AIC under Bayesian framework?

Why we can not apply AIC under Bayesian framework. The AIC is based on the deviance and this latter is based on the likelihood. Now, if the likelihood function is available in closed-form and can...

09 March 2024 510 3 View