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I am trying to transfect an sh-rna in my mammalian cells using lipofectamine 2000 reagent but every time transfection does not completely. Im using 1.5 ug of dna 5ul of lipofectamine and 200ul of...
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I have to clone a cDNA to insert in pcdna3.1. The forward primer generated is having tm 83 and reverse primer has tm 67. I am not getting amplification from normal pcr, gradient pcr, hotstart pcr...
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