I am doing layer by layer coating using chitosan and pectin on low density polyethylene film (LDPE). I want to see antifungal activity ?

The fungus i am using is Mucor hiemalis and i am doing layer by layer coating using chitosan and pectin on LDPE film. I want to see antifungal activity (inhibition zone) of the coated film. The...

03 December 2019 1,825 1 View

My donor and recipint parents are showing amplification with same specific primers. please suggest why?

I am working on marker assisted backcross breeding in rice and during the foreground selection I can see the band in donor as well as recipient parent although it is of different band size. The...

24 April 2019 6,946 3 View

Given a front/side and top views of an engineering drawing image what techniques can be applied to analyze such image and recognize multiple shapes?

Formally, a computer program should be able to scan such an image, perform image processing or any specific treatment on it and produce the followings. 1) The info about multiple geometrical...

09 May 2018 2,390 4 View

What is killing initiation of large scale public health interventions?

Over past few days, while teaching my students, I realized the paradox (individuals paradox, my name for it) in medical teaching, training and management. While our entire research and training is...

05 April 2018 8,209 3 View

Contamination in my genomic prep

I performed a genomic DNA prep today and my 260/280 ratio is 2.10. Is there a way to clean this up such that I don't have to redo the entire protocol (I don't have enough samples at the moment)?...

07 June 2017 1,088 2 View

Can anybody provide me the protocol for pigment extraction from leaves for HPLC analysis

I tried many, but pigments are not extracted

17 February 2016 4,742 4 View

Can you suggest literature review on market strategies in temporary roofing industry?

This is related to corrugated sheeting industry

12 September 2015 9,740 3 View

Which is the best transfection reagent to transfect siRNA in SHSY5Y cells?

I have been trying to transfect siRNA in SHSY5Y cells using lipofectamine 2000 but could not find knockdown of my protein of interest.

12 February 2013 3,451 1 View

What is the best and easiest way of inducing T Cell activation in-vivo?

I intend to study a protein (X) in context to its role during T Cell activation. It doesn't matter whether it is Th1 or Th2. I need some suggestions like the kind of mitogen, chemically induced etc.

05 November 2012 710 6 View

Health Research in tribal population at high altitude.

Tribal populations at high altitude provide an interesting epidemiological window to study human evolution and adaptation. Is research in these un-reached areas still less explored thereby...

01 January 1970 7,920 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View