theoretical framework and conceptual framework?
I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...
07 June 2024 7,292 1 View
I had mammalian cancer cell line cultured and fixed for confocal imaging. While observing I found these particles around cell nuclei stained with dapi. I checked the main culture for bacteria but...
15 May 2024 9,102 2 View
I had mammalian cancer cells cultuted and fixed on confocal slides. I could see these small small bodies stained with dapi. The main culture flask doesnt show any bacterial growth. Is this...
15 May 2024 6,040 3 View
I was running sds page 8% gel and it started running tilted(see the picture attached). Can anyone tell what can be the reason behind gel running like this?
17 January 2024 7,779 0 View
I revived 2 skov3 cell lines together in a t-25 flask. These were stored in -80 for more than 6 months. Their morphology is changed. They look stressed. But can anyone what can be the cause of stress?
22 December 2023 6,799 0 View
theoretical framework ?
28 November 2023 7,396 2 View
I am trying create a mutant plasmid using DpnI digestion. I had put pcr using the mutant primers. But then as I could not do immediate transformation, I kept it to store in -20 degree. Now if I...
08 September 2023 3,621 1 View
I am trying to develop my western blot using an antibody targeting a 17kda protein. It is binding non-specifically and not giving any band(like in the photo). What does it mean is happening? How...
22 August 2023 3,472 4 View
I tried to revive a2780 cells cryopreserved in -80 degrees. Its been more than 12 hours and its still not attaching. What could possibly be happening? What should I do?
21 August 2023 848 4 View
I designed primers which have the tm 69 is and the gc content is 41%. I tried increasing gc content upto 50% but then its increasing primer tm to 72. If I keep gc content 41%, will my primers...
29 June 2023 7,210 2 View
Relativistic space-time is described as a four-dimensional continuum comprising three dimensions of space and one dimension of time. In this framework, space and time are interwoven, forming an...
29 July 2024 5,365 2 View
Referido a las capacidades de los padres para la crianza de sus hijos.
22 May 2024 9,122 0 View
I am studying the continuum mechanics based model of sintering proposed by Olevsky et. al. in the context of spark plasma sintering. First proposition of the model came in 1997:...
16 May 2024 9,329 0 View
What is the difference between conceptual framework and working model while writing the dissertation?
12 May 2024 6,306 1 View
GR and SR are very good theories in the sense that all the assumptions behind them are states clearly, ipenly and elaboratively. Is this a feature of honesty of the author or cleanless of the...
08 May 2024 9,355 4 View
Hello everyone, Thank you for your time and patience in advance! I attempt to develop a pedagogical framework and a competence scale for my PhD project. I have developed the drafts of both. I...
06 May 2024 2,239 3 View
To conduct a bibliometric analysis for a review paper in economic or social science, it is crucial to select the appropriate structure to address the research question. Among the available...
27 April 2024 497 9 View
In an interventional study, is there a criterion for choosing a secondary outcome or a second dependent variable? Should these two variables in the study of medical sciences and especially...
26 April 2024 9,695 1 View
I am working in the area of digital finance, and i want to develop a scale, but there is no pre existing scale for the concerned variable. All the existing measures are taken from national surveys...
23 April 2024 6,007 2 View
compare the post modem perspective by Savikas career construction theory and the African perspective systems theory as framework for career choice.
10 April 2024 2,407 0 View
