How long can we keep pcr in storage before using it for dpnI digestion?

09 September 2023 1 4K Report

I am trying create a mutant plasmid using DpnI digestion. I had put pcr using the mutant primers. But then as I could not do immediate transformation, I kept it to store in -20 degree. Now if I use that pcr after 3 days for DpnI digestion and transformation, will it work?

Michael J. Benedik

It should be fine, I would not expect 3 days at -20 to be an issue.

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