We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used instead of MgCl2 (like PCR) in the LAMP assay?
I do not think that it makes any difference whether you use chloride or sulphate. The important part is the magnesium ions
I do not think that it makes any difference whether you use chloride or sulphate. The important part is the magnesium ions
MgSo4 affects the efficiency of Bst DNA polymerase activity intractiin with dNTPS and primers and also the formation of the magnesium pyrophosphate by product the HNB (hydroxy naphthol blue) colour change and reverse transcription .whille MgCl2 in pcr nesseary to Taq polymerase reaction enzym work
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