Arittra Bhattacharjee

3 Questions 5 Answers 0 Followers

Questions related from Arittra Bhattacharjee

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

05 December 2020 7,831 6 View

Why Magnesium Sulfate is used in Loop-Mediated Isothermal Amplification(LAMP) reaction instead of MgCl2?

We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used...

23 September 2020 7,208 5 View

Can anyone suggest the reason for pink-colored colonies of Burkholderia pseudomallei in MacConkey agar media?

Burkholderia pseudomallei as we know is a non-fermentor of lactose. However, it appears as pink-colored colonies in MacConkey agar media following 48hours of incubation. I have cultured strains...

18 September 2020 7,917 6 View